A new controlled trial involving 120 adults found that those who consumed artificial sweeteners experienced changes in their stools and oral microbiota. The results indicated that two artificial sweeteners, saccharin and sucralose, significantly impacted sugar tolerance. Researchers noted that due to the variability of the microbiome among individuals, artificial sweeteners would affect each person differently. They pointed out that the clinical health effects of the changes that may occur in humans remain unknown and warrant future long-term studies. Both the American Heart Association (AHA) and the American Diabetes Association (ADA) have warned against a diet high in artificial sweeteners. According to Healthline, some experts are concerned that artificial sweeteners could lead to weight gain, partly due to the lack of extensive long-term studies on these sugar alternatives. On the other hand, artificial sweeteners might contribute to weight loss when used as a replacement for high-calorie natural sugars; however, due to limited scientific evidence regarding artificial sweeteners, experts still advise limiting their intake in the diet.